Whole genome sequencing of aureobasidin A adaptors derived from SC5314 during short time exposure to sub-MIC concentration of aureobasidin A
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ABSTRACT: Candida albicans lab strain SC5314 was grown in YPD broth supplemented with sub-MIC concentration (5 ng/ml) of aureobasidin A (AbA) for 24h. The culture was then washed and diluted with distilled water. Approximately 200 cells were spread on YPD agar plate. Randomly 40 colonies were tested with spot assay for tolerance to AbA. 21 colonies were tolerant and were sequenced.
Project description:Cryptococcus neoformans lab strain H99 was exposed to 600ng/ml aureobasidin A. Randomly 30 adaptors were chosen. These adaptors and the wild type strains were sequenced.
Project description:Candida tropicalis clinical isolate #12584 was exposed to auerobasidin A (50ng/ml), Randomly 30 adaptors (T12-T41) were selected. These adaptors as well as the parent were sequenced.
Project description:We exposed Candida parapsilosis clinical isolate #12108 to YPD plate supplemented with 40ng/ml of aureobasidin A. We randomly selected 18 adaptors. We did sequencing of these adaptors.
Project description:We treated C. albicans reference strain SC5314 with 20ng/ml of aureobasidin A, and obtained two mutants which were resistant to aureobasidin A. Then we did whole genome sequencing of these two mutants.
Project description:Candida tropicalis clinical isolate #12584 was spread on YPD plate supplemented with 40ng/ml of aureobasidin A. Randomly 18 adaptors were selected. Eight adaptors obtained tolerance. We sequenced the tolerant adaptors.
Project description:We exposed Candida albicans reference strain SC5314 to different drugs (flucytosine, aureobasidin A, amphotericin B), and sequenced some of the adaptors.
Project description:Candida albicans isolate YJB-T490 was spread on YPD plates supplemented with fluconazole. The plates were incubated at 37C. 54 adaptors were sequenced.
Project description:Candida albicans lab strain SC53114 was used to obtain fluconazole-tolerant adaptors. 53 adaptors as well as the parent strain were sequenced.
Project description:Candida albicans lab strain SC5314 was daily passaged in YPD broth supplemented with fluconazole. Some fluconazole-resistant and some fluconazole-tolerant adaptors were sequenced.