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Transcription profiling by tiling array of combinations of Rrp6p and Dis3p catalytic mutants to investigate extensive degradation of RNA precursors by the exosome in yeast

ABSTRACT: The exosome is a complex involved in the maturation of rRNA and sn-snoRNA, the degradation of short lived non-coding RNAs and in the quality control of RNAs produced in mutants. It contains two catalytic subunits, Rrp6p and Dis3p, whose specific functions are not fully understood. We analyzed the transcriptome of combinations of Rrp6p and Dis3p catalytic mutants by high-resolution tiling arrays. We show that Dis3p and Rrp6p have both overlapping and specific roles in degradation of distinct classes of substrates. We found that transcripts derived from more than half of intron-containing genes are degraded before processing. Surprisingly, we also show that the exosome degrades large amounts of tRNA precursors despite the absence of processing defects. These results underscore the notion that large amounts of RNAs produced in wild type cells are discarded before entering functional pathways, suggesting that kinetic competition with degradation proofreads the efficiency and accuracy of processing.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Zhenyu Xu

PROVIDER: E-MTAB-1246 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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