Single-cell RNA (10X Chromium) of naked mole rate DMBA/TPA treated or control back skin and matched livers (CRUK B) 2022

ABSTRACT: We generated single cell transcriptomes from full thickness skin biopsies in naked mole-rat to quantify the skin cell types found in this species (control samples). To study if and how naked mole-rat skin changes upon exposure to a carcinogen, we performed a classical two-stage skin carcinogenesis experiment traditionally performed in mice, wherein cancer is initiated by a single application of 7,12-dimethylbenz[a]-anthracene (DMBA) followed by repeated treatment with 12-O-tetradecanoylphorbol-13-acetate (TPA) to drive cell proliferation. After 12 weeks, full thickness skin biopsies were collected and used to generate single cell transcriptomes (treatment samples).

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Heterocephalus glaber

SUBMITTER: Pablo Baeza Centurion 

PROVIDER: E-MTAB-12461 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress