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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of mouse erythroleukemia cells following activation of Gata1-ER or PU.1-ER transgenes

ABSTRACT: 17b-Estradiol added to MEL cells expressing Gata1-ER or PU.1-ER transgenes to stimulate either erythropoietic Gata-1 dependent or myeloid PU.1 dependent gene espression in different time points

ORGANISM(S): Mus musculus

SUBMITTER: Tomas Stopka

PROVIDER: E-MTAB-125 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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PU.1 activation relieves GATA-1-mediated repression of Cebpa and Cbfb during leukemia differentiation.

Burda Pavel P Curik Nikola N Kokavec Juraj J Basova Petra P Mikulenkova Dana D Skoultchi Arthur I AI Zavadil Jiri J Stopka Tomas T

Molecular cancer research : MCR 20091013 10

Hematopoietic transcription factors GATA-1 and PU.1 bind each other on DNA to block transcriptional programs of undesired lineage during hematopoietic commitment. Murine erythroleukemia (MEL) cells that coexpress GATA-1 and PU.1 are blocked at the blast stage but respond to molecular removal (downregulation) of PU.1 or addition (upregulation) of GATA-1 by inducing terminal erythroid differentiation. To test whether GATA-1 blocks PU.1 in MEL cells, we have conditionally activated a transgenic PU. ...[more]

PMID: 19825991

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