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Single-cell RNA-seq of hESC-derived, differentiating cardiac cells on day 2, 3 and 8; control and PD0325901-treated.

ABSTRACT: The role of FGF-MEK-ERK signalling pathway during embryonic heart development has not been fully elucidated. Here, we inhibited the pathway for 1 day using PD0325901, a MEK inhibitor, at the lateral plate mesoderm stage during cardiac differentiation of human embryonic stem cells. Cells were collected on day 2 (before PD0325901 administration), day 3 and day 8 to determine the effect of a transient FGF-MEK-ERK pathway modulation on the cardiac cell fate choice.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Karin Farkas

PROVIDER: E-MTAB-12619 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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