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Single cell RNA sequencing of CellTagging lung organoids

ABSTRACT: Here, we used a lentiviral-based barcoding method (CellTagging) to lineage trace progenitor cells in a human lung organoid model. 21-day cultures were transduced for 7 days, fluorescence activated cell sorted for GFP+ expression, and regrown as sparsely plated cells for additional 30 days before sequencing. Cultures were grown in serum-free basal media supplemented with FGF-10, A-8301, NOGGIN, and Y-27632 (‘DSI media’).

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Jason Spence

PROVIDER: E-MTAB-12753 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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