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CUT&Tag of mouse primary B cells transduced Pbx1 overexpression plasmid.

ABSTRACT: We performed the cleavage under targets and tagmentation (Cut & tag) assay followed by sequencing enriched DNA fragments to reveal the direct downstream targets of Pbx1. Firstly, we overexpressed Pbx1b with Pbx1b-IRES-GFP retrovirus in murine peripheral B cells to ensure the yields of DNA fragments. CUT & tag libraries were generated following instructions of the manufacturer’s protocol (Vazyme; cat TD901-01) and the Pbx1 antibody (CST; cat 4342) was used for signal enrichment.

INSTRUMENT(S): HiSeq X Ten

ORGANISM(S): Mus musculus

SUBMITTER: Nan Shen

PROVIDER: E-MTAB-12769 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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