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Unknown,Transcriptomics,Genomics,Proteomics

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Bulk mRNA Seq CAR.CCR7 vs. CAR

ABSTRACT: The study aimed to elucidate the transcriptional changes introduced by genetic engineering of CAR T cells to stably express the homeostatic chemokine receptor CCR7. In vitro killing assays hinted to a higher cytolytic capacity that could be tied to an altered cytoskeletal rearrangement. Human CAR and CAR.CCR7 T cells were generated by retroviral transduction and enriched by FACS at the end of the expansion phase (day 10), either without or with a previous over-night co-culture with target Jeko1 tumor cells that express the CAR target antigen CXCR5. Enriched cells were immediately processed for bulk mRNA Seq

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Maria Zschummel

PROVIDER: E-MTAB-12778 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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