Single cell CITE-seq and VDJ sequencing of CD8+ central memory blood T cells (TCM) and their in-vivo matured tissue-resident memory-like T cells (TRM)
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ABSTRACT: The aim of the experiment was to understand the clonal relationship between CD8+ central memory blood T cells (TCM) and the in-vivo matured TCM tissue-resident memory-like T cells (TRM) on day 14. TCM were FACS sorted from human PBMCs (n=5). Half of the cells were processed on day 1 and half of the cells of each donor were matured into TRM with anti-CD3, IL-15 and TGF beta for 14 days. Both day 1 TCM and matured day 14 TRM-like cells were further processed using the 5´10X genomics workflow.
INSTRUMENT(S): 10x Genomics Chromium Controller, Illumina NovaSeq 6000
ORGANISM(S): Homo sapiens
SUBMITTER: Wenning Zheng
PROVIDER: E-MTAB-12840 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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