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Gene Expression Analysis of the Short Term Ingestion of Titanium Dioxide Nanofibers

ABSTRACT: Titanium dioxide nanofiber (TDNF) is widely used in the manufacture of various household products including cosmetics. As a result, there is a possibility of negative impact on human health. The goal of the present study is to investigate the short-term effects of titanium dioxide nanofiber ingestion of male Sprague Dawley rats. TDNF was fabricated via electrospinning method, followed by dissolution in water. Six- to seven-week-old male Sprague Dawley rats were exposed to a total of 0, 40, and 60 ppm of TDNF for 2 weeks via oral gavage. Lungs and other tissues were autopsied and excised immediately frozen in liquid nitrogen and stored at -80 deg C until analysis. Total RNA was converted to single-stranded sense-target, fragmented, labeled, and hybridized to the Affymetrix Rat Gene ST 2.0 microarray according to the manufacturer’s protocol.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Worlanyo Eric Gato

PROVIDER: E-MTAB-13260 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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