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Chromatin immunoprecipitation of mouse MIN6 pancreatic beta cells to identify Pdx1 targets

ABSTRACT: The aim of this experiment was to use highthroughput chromatin immunoprecipitation followed by hybridization to promoter microarrays to obtain a comprehensive list of sites in the genome that are physically occupied by Pdx1. Chromatin was prepared from Min6 insulinoma cells and immunoprecipitated with Pdx1 or control antiserum. The precipitated chromatin was then purified, amplified and directly sequenced using Illumina technology.

ORGANISM(S): Mus musculus

SUBMITTER: Mira Sachdeva

PROVIDER: E-MTAB-134 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Pdx1 (MODY4) regulates pancreatic beta cell susceptibility to ER stress.

Sachdeva Mira M MM Claiborn Kathryn C KC Khoo Cynthia C Yang Juxiang J Groff David N DN Mirmira Raghavendra G RG Stoffers Doris A DA

Proceedings of the National Academy of Sciences of the United States of America 20091023 45

Type 2 diabetes mellitus (T2DM) results from pancreatic beta cell failure in the setting of insulin resistance. Heterozygous mutations in the gene encoding the beta cell transcription factor pancreatic duodenal homeobox 1 (Pdx1) are associated with both T2DM and maturity onset diabetes of the young (MODY4), and low levels of Pdx1 accompany beta cell dysfunction in experimental models of glucotoxicity and diabetes. Here, we find that Pdx1 is required for compensatory beta cell mass expansion in r ...[more]

PMID: 19855005

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