RNA-seq of paired oocytes and granulosa cells from 3M and 12M old mice after natural and superovulation
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ABSTRACT: Oocytes and granulosa cells were collected after natural and superovulation in young (3 month-old) and old (12 month-old) mice while conserving pairing information between oocyte and granulosa cells from each cumulus-oocyte-complex (COC). This experimental design allows for the analysis of the effects of ageing and / or superovulation on the transcriptome of COCs. The naming convention for samples is as follows: [ovulation, NO/SO][age, 3M/12M][cell, OC/GC][COC number], meaning that NO12MOC01 and NO12MGC01 are from the same COC. Oocyte and granulosa cell samples were processed for full-length cytoplasmic RNA amplification using slightly modified SMART-seq2 protocol. After amplification of cDNA and library preparation, samples were sequenced on NextSeq 550 using Single-Ended 75bp High-Output kit.
INSTRUMENT(S): NextSeq 550
ORGANISM(S): Mus musculus
SUBMITTER: Angela Gonçalves
PROVIDER: E-MTAB-13479 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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