Extensive Differential DNA Methylation in Bovine Tuberculosis Skin Test Positive and Skin Test Negative Cattle

ABSTRACT: Bovine tuberculosis (bTB), caused by Mycobacterium bovis, represents a significant issue for the global agriculture industry as well as for human health. Epigenetic modifications can alter the course of the immune response and differentially methylated regions (DMRs) could contribute to the failure of current generation tests to detect all TB-infected cattle. Whole Genome Bisulphite Sequencing (WGBS) was used to profile DNA methylation levels from peripheral blood of cattle naturally infected with M. bovis (positive for the single intradermal comparative tuberculin test (SICTT) and/or the interferon-γ release assay (IGRA) compared to negative controls [n=8/group, total of 16 WGBS libraries]. Although overall methylation profiles were similar across the genome, 224 DMRs and 159 Differential

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Bos taurus

SUBMITTER: Alia Parveen 

PROVIDER: E-MTAB-13487 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress