Unknown,Transcriptomics,Genomics,Proteomics

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MRNA-Seq of Myriophyllum spicatum exposed to low-effect concentrations of the herbicide bentazone and the pharmaceutical atorvastatin against untreated control groups

ABSTRACT: Within this study, the non-model organism Myriophyllum spicatum was used to evaluate ecotoxic modes of action at the gene expression level. M. spicatum was exposed to low-effect concentrations of bentazone and atorvastatin in a shortened and modified version of the OECD guideline test No. 239, followed by RNA extraction of the shoot tip tissue and a subsequent RNA-seq analysis utilizing a de novo assembly of the transcriptome. While the herbicide bentazone is an inhibitor of photosynthesis, the widely spread pharmaceutical atorvastatin acts as an inhibitor of the hydroxymethylglutaryl-CoA reductase and thus the isoprenoid biosynthesis. The aim of this study was to determine molecular fingerprints and biomarkers distinguishing these distinct modes of action at a molecular level.

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Myriophyllum spicatum

SUBMITTER: Jost Hanfland

PROVIDER: E-MTAB-13702 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Testing for toxicity in a number of aquatic organisms is necessary for risk assessments of substances. Yet, aquatic larvae of the so-called EPT (Ephemeroptera, Plecoptera, Trichoptera) taxa are regularly exposed to several environmental contaminants and have been demonstrated to be extremely vulnerable to a variety of environmental pollutants. These results show that existing toxicity testing can result in an underestimating of the risk for EPT taxa and that more toxicity data using EPT taxonomic representatives are needed. Unfortunately, there is a dearth of standardized test techniques and scant published data, particularly for European EPT species. Our study's objective was to create a testing framework for a variety of endpoints in the mayfly Cloeon dipterum. Due to its high prevalence in local waterbodies and its brief lifecycle of a few weeks in the right environmental conditions, C. dipterum was selected as the test organism. To this end, two chronic toxicity tests with semi-static test design and two media renewal per week were performed. Small larvae in the stage L3 based on the wing pad development described by Cianciara (1976) were used in the tests. Four replicates per test concentration and control containing five individuals per replicate were installed. The emergence was determined daily on working days. The tests were conducted at a temperature of 20 °C (± 1 °C) and the illumination was < 1 µE m2s 1 with a light and dark rhythm of 16:8 h. The physico-chemical parameters pH, oxygen concentration, and oxygen saturation were measured using the multiparameter device WTW Multi 1970i at test start, throughout each media renewal, and at test end. Continuous temperature measurements were taken, and weekly lighting assessments were made. Fipronil was administered to C. dipterum larvae in the long-term exposure experiment at nominal concentrations ranging from 0.038 to 0.60 g/L. This test was conducted for 38 days until all larvae had emerged. In the short-term exposure experiment, C. dipterum larvae were exposed to Fipronil in nominal concentrations between 0.038 and 0.30 µg/L for a test duration of seven days. At test end, the larvae were sampled for transcriptome analysis.

Dataset Information

MRNA-Seq of Myriophyllum spicatum exposed to low-effect concentrations of the herbicide bentazone and the pharmaceutical atorvastatin against untreated control groups

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