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Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptome-wide mapping of hnRNP C and U2AF65 using iCLIP

ABSTRACT: iCLIP experiment to assess the binding of the highly abundant nuclear RNA-binding protein hnRNP C and core splicing factor U2AF65 on a genomic scale. To investigate how both proteins compete for binding at a subset of sites, U2AF65 iCLIP experiments were performed from both HNRNPC knockdown and control HeLa cells.

ORGANISM(S): Homo sapiens

SUBMITTER: Kathi Zarnack

PROVIDER: E-MTAB-1371 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Direct competition between hnRNP C and U2AF65 protects the transcriptome from the exonization of Alu elements.

Zarnack Kathi K König Julian J Tajnik Mojca M Martincorena Iñigo I Eustermann Sebastian S Stévant Isabelle I Reyes Alejandro A Anders Simon S Luscombe Nicholas M NM Ule Jernej J

Cell 20130101 3

There are ~650,000 Alu elements in transcribed regions of the human genome. These elements contain cryptic splice sites, so they are in constant danger of aberrant incorporation into mature transcripts. Despite posing a major threat to transcriptome integrity, little is known about the molecular mechanisms preventing their inclusion. Here, we present a mechanism for protecting the human transcriptome from the aberrant exonization of transposable elements. Quantitative iCLIP data show that the RN ...[more]

PMID: 23374342

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