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RNA-seq investigating the influence of astrocytes on developing cortical neurons

ABSTRACT: Astrocytes are implicated in neuronal development, particularly excitatory synaptogenesis, but their genome-wide impact is unclear. Using cell-type specific RNA-seq we show that cortical astrocytes induce widespread transcriptomic changes in developing cortical neurons. Rat cortical neurons were maintained in the presence or absence of mouse astrocytes, RNA-seq performed, and mixed-species RNA-seq reads sorted according to species. Cultures were also treated with TTX to abolish neuronal firing activity, to investigate the effects of the presence or absence activity-dependent signalling.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Rattus norvegicus

SUBMITTER: Owen Dando

PROVIDER: E-MTAB-14070 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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