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Identifying crossover locations in an Arabidopsis thaliana Col x Ler F2 population using genotyping by sequencing

ABSTRACT: Genomic DNA from 55 Col x Ler F2 individuals was extracted using CTAB and used to generate sequencing libraries as described (Rowan et al; Yelina et al), with the following modifications. DNA was extracted from 3 rosette leaves of 5 week old plants and 150 ng of DNA used as input for each library. DNA shearing was carried out for 20 minutes at 37°C with 0.4U of DNA Shearase (Zymo research). The barcoded adapters used for library construction are listed in Rowan et al. Each set of 96 libraries was sequenced on one lane of an Illumina NextSeq500 instrument (300-cycle Mid Output run). Sequencing data was analysed to identify crossovers as previously reported, using the TIGER pipeline. The same gDNA was pooled into two groups (pool1 = F2_1 to F2_4; pool2 = F2_5 to F2_55) and sequenced with Nanopore PromethION, which is available at E-MTAB-14367

INSTRUMENT(S): Illumina NovaSeq X

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Kyuha Choi

PROVIDER: E-MTAB-14380 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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