Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Differential expression of S. coelicolor M145 vs. an abrC3 mutant in NB

ABSTRACT: Differential expression data set of S. coelicolor M145 and a abrC3 mutant cultured in NB. RNA samples were collected at 36, 48 and 60 hours following cDNA synthesis and labeling. The hybridization was conducted using DNA 104K microarrays (Surrey) using gDNA from S. coelicolor M145 as reference.

ORGANISM(S): Streptomyces coelicolor A3(2)

SUBMITTER: Emma Laing

PROVIDER: E-MTAB-1453 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Deciphering the regulon of Streptomyces coelicolor AbrC3, a positive response regulator of antibiotic production.

Rico Sergio S Santamaría Ramón I RI Yepes Ana A Rodríguez Héctor H Laing Emma E Bucca Giselda G Smith Colin P CP Díaz Margarita M

Applied and environmental microbiology 20140207 8

The atypical two-component system (TCS) AbrC1/C2/C3 (encoded by SCO4598, SCO4597, and SCO4596), comprising two histidine kinases (HKs) and a response regulator (RR), is crucial for antibiotic production in Streptomyces coelicolor and for morphological differentiation under certain nutritional conditions. In this study, we demonstrate that deletion of the RR-encoding gene, abrC3 (SCO4596), results in a dramatic decrease in actinorhodin (ACT) and undecylprodiginine (RED) production and delays morp ...[more]

PMID: 24509929

Similar Datasets

S. coelicolor M145 SMM medium time course study

Project description:This SuperSeries is composed of the following subset Series: GSE22394: S. coelicolor M145 SMM medium time course study (Culture # 1) GSE22395: S. coelicolor M145 SMM medium time course study (Culture # 2) GSE22397: S. coelicolor M145 SMM medium time course study (Culture # 3) Refer to individual Series

2010-10-25 | E-GEOD-22399 | biostudies-arrayexpress

Streptomyces coelicolor cells during various developmental stages: wildtype M145 vs. ÎSCO0204.

Project description:Global transcriptional profiling of the SCO0204 null mutant of Streptomyces coelicolor M145 in comparison to the wildtype M145. Goal was to determine the role of SCO0204.

2016-04-14 | E-MTAB-4597 | biostudies-arrayexpress

S. coelicolor M752 SMM medium time course study

Project description:Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarrays to investigate the temporal transcriptome profiles of S. coelicolor A3(2) M752 mutant strain, which has an in frame deletion of scbR (SCO6265) and compare it to the wild-type M145 strain. scbR encondes a gamma-butyrolactone binding protein (Takano, E., Chakraburtty, R., Nihira, T., Yamada, Y., and Bibb, M. A complex role for the gamma-butyrolactone SCB1 in regulating antibiotic production in Streptomyces coelicolor A3(2). Mol. Microbiol. 2001 41(5), 1015-1028). The time-course study involved analysis of 8 samples (14hr, 15hr, 16hr, 17hr, 19hr, 20hr, 22hr, 39hr). Sample 22hr was analyzed on duplicate arrays (2 technical replicates). Genomic DNA of S. coelicolor wild type (M145) was used as a reference for all the arrays. cDNA was labeled with Alexa647 and genomic DNA was labeled with Cy3.

2010-10-25 | E-GEOD-22398 | biostudies-arrayexpress

S. coelicolor LY2517 R5- medium time course study (Culture # 1)

Project description:Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarrays to investigate the temporal transcriptome profiles of S. coelicolor A3(2) deltaSCO2517::kan mutant strain (LY2517) and compare it to the wild-type M145 strain. SCO2517 is a putative two-component system response regulator. The time-course study involved analysis of 6 samples (15h, 22h, 24h, 26h, 35h, 43h). All samples were analyzed on duplicate arrays (2 technical replicates). Genomic DNA of S. coelicolor wild type (M145) was used as a reference for all the arrays. cDNA was labeled with Alexa647 and genomic DNA was labeled with Cy3.

2012-12-19 | E-GEOD-21809 | biostudies-arrayexpress

S. coelicolor LY2517 R5- medium time course study (Culture # 2)

Project description:Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarrays to investigate the temporal transcriptome profiles of S. coelicolor A3(2) deltaSCO2517::kan mutant strain (LY2517) and compare it to the wild-type M145 strain. SCO2517 is a putative two-component system response regulator. The time-course study involved analysis of 13 samples (14h, 16h, 18h, 20h, 22h, 24h, 26h, 28h, 30h, 32h, 34h, 37h, 39h). Genomic DNA of S. coelicolor wild type (M145) was used as a reference for all the arrays. cDNA was labeled with Alexa647 and genomic DNA was labeled with Cy3.

2012-12-19 | E-GEOD-21810 | biostudies-arrayexpress

S. coelicolor Wild Type Time-course Study in R5- medium (Culture#1)

Project description:Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarrays to investigate the temporal transcriptome profiles of S. coelicolor A3(2) M145 wild type and disruption mutants of regulatory genes (afsS and absA1) known to affect antibiotic biosynthesis. Keywords: Time course The time-course study involved analysis of 6 samples (15h, 22h, 24h, 26h, 35h, 43h). Of these, 15h and 22h were analyzed in duplicate arrays. Genomic DNA of S. coelicolor wild type (M145) was used as a reference for all the arrays. cDNA was labeled with Alexa647 and genomic DNA was labeled with Cy3.

2010-06-10 | E-GEOD-8084 | biostudies-arrayexpress

S. coelicolor YSK3654 R5- medium time course study

Project description:Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarrays to investigate the temporal transcriptome profiles of S. coelicolor A3(2) deltaSCO3654::apr mutant strain (YSK3654) and compare it to the wild-type M145 strain. SCO3654 is a putative two-component system sensor kinase. The time-course study involved analysis of 9 samples (10h, 13h, 16h, 19h, 22h, 25h, 31h, 35h, 37h). All samples were analyzed on duplicate arrays (2 technical replicates). Genomic DNA of S. coelicolor wild type (M145) was used as a reference for all the arrays. cDNA was labeled with Alexa647 and genomic DNA was labeled with Cy3.

2010-10-25 | E-GEOD-21807 | biostudies-arrayexpress

S. coelicolor M145 SMM medium time course study (Culture # 2)

2012-12-19 | E-GEOD-22395 | biostudies-arrayexpress

S. coelicolor WL6268 R5- medium time course study

Project description:Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarrays to investigate the temporal transcriptome profiles of S. coelicolor A3(2) deltaSCO6268::kan mutant strain (WL6268) and compare it to the wild-type M145 strain. SCO6268 is a putative histidine kinase. The time-course study involved analysis of 7 samples (12h, 16h, 18h, 19h, 21h, 25h, 33h). Samples (19h, 21h, 25h, 33h) were analyzed on duplicate arrays (2 technical replicates). Sample 16h was analyzed on triplicate arrays (3 technical replicates). Genomic DNA of S. coelicolor wild type (M145) was used as a reference for all the arrays. cDNA was labeled with Alexa647 and genomic DNA was labeled with Cy3.

2010-10-25 | E-GEOD-21808 | biostudies-arrayexpress

S. coelicolor Wild Type Time-course Study in R5- medium (Culture#2)

Project description:Antibiotic biosynthesis in Streptomyces species is controlled by a complex genetic and biochemical network of global and pathway specific regulators. Details of their precise interactions in mediating temporal and spatial expression of secondary metabolite genes remain poorly defined. In this study, we employed whole-genome microarrays to investigate the temporal transcriptome profiles of S. coelicolor A3(2) M145 wild type and disruption mutants of regulatory genes (afsS and absA1) known to affect antibiotic biosynthesis. Keywords: Time course The time-course study involved analysis of 9 samples (10h, 13h, 16h, 19h, 22h, 25h, 31h, 35h, 37h). Of these, the 16h sample was analyzed on three arrays (3 technical replicates). All other samples were analyzed on duplicate arrays (2 technical replicates). Genomic DNA of S. coelicolor wild type (M145) was used as a reference for all the arrays. cDNA was labeled with Alexa647 and genomic DNA was labeled with Cy3.

2010-06-11 | E-GEOD-8086 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data