Identification of tens of thousands of novel gene loci in the human and mouse genomes through targeted full-length long-read RNA sequencing

ABSTRACT: The GENCODE project is a long-term international effort to produce a comprehensive and accurate map of genes and transcripts for the human and mouse genomes. While the annotation of protein-coding genes is nearly complete, long non-coding RNAs (lncRNAs) remain poorly characterized, with existing catalogs lacking consistency and experimental support. To address this, GENCODE used a targeted RNA sequencing approach to capture RNA from various human and mouse tissues, employing advanced sequencing technologies (ONT, PacBio, and Illumina). This resulted in the prediction of around half a million transcript models for both species. GENCODE then re-engineered its curation pipeline to handle this data, leading to the annotation of 16,817 new human genes (132,049 transcripts) and 22,210 new mouse

INSTRUMENT(S): Illumina HiSeq 2500, MinION, Sequel II

ORGANISM(S): Homo sapiens

SUBMITTER: Sílvia Carbonell-Sala 

PROVIDER: E-MTAB-14562 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress