Whole transcriptome RNAseq of the effect of photobiomodulation after spinal cord injury

ABSTRACT: The purpose of the experiment was to determine whole transcriptome changes after spinal cord injury (SCI) to understand how photobiomodulation promotes neuroprotection and functional recovery. SCI was performed at the thoracic (T) level T8, by crushing the dorsal columns using a calibrated watchmaker's forceps. Animals were then treated with photobiomodulation (PBM; 660nm wavelength) or sham control light, within 15 minutes of the injury. PBM or sham-treatment was treatment directed at the lesion site for 1 minute duration every 24hr for the first 3 days. Animals were then killed, tissues harvested and the RNA was extracted using RNEasy lipid tissue mini kit (Qiagen) according to the manufacture's instructions. Whole genome sequencing was outsourced to Qiagen RNA Sequencing Services (Germa

INSTRUMENT(S): none, non, Illumina

ORGANISM(S): Rattus norvegicus

SUBMITTER: Zubair Ahmed 

PROVIDER: E-MTAB-14593 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress