Continuous map of early hematopoietic stem cell differentiation across human lifetime

ABSTRACT: We employed CITE-Seq profiling of 62 277 high-quality FACS-enriched human bone marrow hematopoietic stem and progenitor cells (HSPCs) from 15 healthy donors representing young, mid and old age groups, using the BD Rhapsody platform. Our targeted gene panel consisting of 596 genes was supplemented with 46 cell-surface proteins to cover the most relevant markers of early HSPC differentiation and cell cycle status. We demonstrate a molecular map of early human hematopoietic stem cell (HSC) differentiation and provide evidence of early branching points into lineage trajectories across different age groups. Our data shows that the HSC branched into two main lineages, with the first branching point giving rise to megakaryocytic and erythroid progenitors (MKP/ERP), followed by lymphoid and myelo

INSTRUMENT(S): BD Rhapsody Express Instrument, NextSeq 550, Illumina NovaSeq 6000, NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Hana Komic 

PROVIDER: E-MTAB-14596 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress