Methylation-sensitive SMiLE-seq sequencing data (illumina) of human transcription factors profiled in the Codebook/GRECO-BiT collaboration

ABSTRACT: We assayed 114 human transcription factors (TFs) for DNA binding using methylation-sensitive selective microfluidics-based ligand enrichment followed by sequencing (meSMiLE-seq). Across 23 experiments, we identified DNA binding sites for 48 TFs. Among these, 13 showed aversion towards methylated DNA, while 11 exhibited either a preference for methylated DNA or an alternative binding site including the modification. The DNA binding for the remaining TFs was unaffected by DNA methylation under the conditions of the assay.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): synthetic construct

SUBMITTER: Antoni Gralak 

PROVIDER: E-MTAB-14597 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress