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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of human mammary epithelial cell line MCF10A with RMRP/CCDC107/ARHGEF39-targeted CRISPR edits vs. scramble controls

ABSTRACT: The purpose of these experiments was to validate the role of a frequently mutated regulatory element bound by TOP2B using a functional genomics approach. Specifically, CRISPR/Cas9 was employed to selectively target the promoter of RMRP and non-coding regions of CCDC107 and ARHGEF39.

INSTRUMENT(S): Zymo Quick-RNA Microprep Kit (Zymo Research R1051), 12-well plates (Sarstedt 83.3921), 24-Well Plates, RNA Qubit (Invitrogen Q32852), NextSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER: Christian Lee

PROVIDER: E-MTAB-14611 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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