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RNA-seq analyses of gene expression in the microsclerotia of Verticillium dahliae


ABSTRACT: Verticillium dahliae is a soilborne fungus that causes wilt disease in plants. The microsclerotia of V. dahliae produce infectious hyphae that give rise to primary infections. In this study, RNA-seq libraries were prepared from microsclerotia (MS)-producing cultures of V. dahliae (ave = 52.23 million reads), and those not producing microsclerotia (NoMS, ave = 50.58 million reads) and analyzed for differential gene expression.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Verticillium dahliae

SUBMITTER: Dongquan Chen 

PROVIDER: E-MTAB-1581 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RNA-seq analyses of gene expression in the microsclerotia of Verticillium dahliae.

Duressa Dechassa D   Anchieta Amy A   Chen Dongquan D   Klimes Anna A   Garcia-Pedrajas Maria D MD   Dobinson Katherine F KF   Klosterman Steven J SJ  

BMC genomics 20130909


<h4>Background</h4>The soilborne fungus, Verticillium dahliae, causes Verticillium wilt disease in plants. Verticillium wilt is difficult to control since V. dahliae is capable of persisting in the soil for 10 to 15 years as melanized microsclerotia, rendering crop rotation strategies for disease control ineffective. Microsclerotia of V. dahliae overwinter and germinate to produce infectious hyphae that give rise to primary infections. Consequently, microsclerotia formation, maintenance, and ger  ...[more]

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