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Transcriptional profiling of 7SK knockdown in mouse embryonic stem cells

ABSTRACT: Transcriptome profiling of mouse embryonic stem cells following antisense knockdown of the non-coding RNA 7SK.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Mus musculus

SUBMITTER: Paul Bertone

PROVIDER: E-MTAB-1585 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The non-coding snRNA 7SK controls transcriptional termination, poising, and bidirectionality in embryonic stem cells.

Castelo-Branco Gonçalo G Amaral Paulo P PP Engström Pär G PG Robson Samuel C SC Marques Sueli C SC Bertone Paul P Kouzarides Tony T

Genome biology 20130101 9

<h4>Background</h4>Pluripotency is characterized by a unique transcriptional state, in which lineage-specification genes are poised for transcription upon exposure to appropriate stimuli, via a bivalency mechanism involving the simultaneous presence of activating and repressive methylation marks at promoter-associated histones. Recent evidence suggests that other mechanisms, such as RNA polymerase II pausing, might be operational in this process, but their regulation remains poorly understood.<h ...[more]

PMID: 24044525

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