Single-cell RNAseq of hiPSC derived alveolar-like lung organoids upon H3N2 infection

ABSTRACT: hiPSC from 2 different donors were differentiated into alveolar-like organoids (iPSC-aLOs) as previously described (DOI: 10.1038/s41596-019-0220-0). iPSC-aLOs were either kept in \"dome\" culture embedded in extracellular matrix or inverted in apical-out conformation to allow influenza H3N2 infection. Apical-out iPSC-aLOs were exposed or not to H3N2. At 24hs post-infection iPSC-aLOs were collected and dissociated into single cells. Samples were multiplexed using membrane lipid barcoding and we followed 10X Genomics instructions from single cell encapsulation to NGS. This data set is useful to compare iPSC-aLOs cell composition both in dome and apical-out culture conditions and iPSC-aLOs response to influenza virus H3N2 infection at a single timepoint of 24hs post-infection at MOI =1. Each

INSTRUMENT(S): Illumina NovaSeq 6000

ORGANISM(S): Homo sapiens

SUBMITTER: Valeria Fernandez Vallone 

PROVIDER: E-MTAB-15980 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress