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Transcription profiling of Legionella pneumophila wild type vs fleR mutant at OD 4.0

ABSTRACT: Comparison of Lp Paris fleR mutant against wt in exponential phase (OD 4.0). We investigated the role of FleQ, FleR, RpoN, and FliA on the regulation of the expression of transmissive traits in L. pneumophila strain Paris at different time points (replicative phase, early transmissive phase, late transmissive phase). Bonferroni adjustment,

ORGANISM(S): Legionella pneumophila

SUBMITTER: Christiane ALBERT

PROVIDER: E-MTAB-164 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription profiling of Legionella pneumophila wild type vs fleQ mutant at OD 3.3

Project description:Comparison of Lp Paris fleQ mutant against wt in exponential phase (OD 3.3). We investigated the role of FleQ, FleR, RpoN, and FliA on the regulation of the expression of transmissive traits in L. pneumophila strain Paris at different time points (replicative phase, early transmissive phase, late transmissive phase).

2009-12-02 | E-MTAB-169 | biostudies-arrayexpress

Transcription profiling of Legionella pneumophila wild type vs fleQ mutant at OD 2.6

Project description:Comparison of Lp Paris fleQ mutant against wt in exponential phase (OD 2.6). We investigated the role of FleQ, FleR, RpoN, and FliA on the regulation of the expression of transmissive traits in L. pneumophila strain Paris at different time points (replicative phase, early transmissive phase, late transmissive phase).

2009-12-02 | E-MTAB-170 | biostudies-arrayexpress

Transcription profiling of Legionella pneumophila wild type vs rpoN mutant at OD 2.6

Project description:Comparison of Lp Paris wt against rpoN mutant in exponential phase (OD 2.6). We investigated the role of FleQ, FleR, RpoN, and FliA on the regulation of the expression of transmissive traits in L. pneumophila strain Paris at different time points (replicative phase, early transmissive phase, late transmissive phase).

2009-12-02 | E-MTAB-171 | biostudies-arrayexpress

Transcription profiling of Legionella pneumophila wild type vs fleQ mutant at OD 4.0

Project description:Comparison of Lp Paris fleQ mutant against wt OD 4.0. We investigated the role of FleQ, FleR, RpoN, and FliA on the regulation of the expression of transmissive traits in L. pneumophila strain Paris at different time points (replicative phase, early transmissive phase, late transmissive phase).

2009-12-02 | E-MTAB-167 | biostudies-arrayexpress

Transcription profiling of Legionella pneumophila wild type vs fleQ mutant at OD 3.9

Project description:Comparison of Lp Paris fleQ mutant against wt at OD 3.9. We investigated the role of FleQ, FleR, RpoN, and FliA on the regulation of the expression of transmissive traits in L. pneumophila strain Paris at different time points (replicative phase, early transmissive phase, late transmissive phase).

2009-12-02 | E-MTAB-168 | biostudies-arrayexpress

Transcription profiling of Legionella pneumophila wild type vs rpoN mutant at OD 3.9

Project description:Comparison of Lp Paris wt against rpoN mutant at late exponential phase (OD 3.9). We investigated the role of FleQ, FleR, RpoN, and FliA on the regulation of the expression of transmissive traits in L. pneumophila strain Paris at different time points (replicative phase, early transmissive phase, late transmissive phase).

2009-12-02 | E-MTAB-173 | biostudies-arrayexpress

Transcription profiling of Legionella pneumophila wild type vs rpoN mutant at OD 4.0

Project description:Comparison of the Lp Paris wt against the rpoN mutant at late exponential/early stationary phase (OD 4.0). We investigated the role of FleQ, FleR, RpoN, and FliA on the regulation of the expression of transmissive traits in L. pneumophila strain Paris at different time points (replicative phase, early transmissive phase, late transmissive phase).

2009-12-02 | E-MTAB-174 | biostudies-arrayexpress

Transcription profiling of Legionella pneumophila wild type vs rpoN mutant at OD 3.3

Project description:Comparison between Lp Paris wt and rpoN mutant in exponential phase (OD 3.3). We investigated the role of FleQ, FleR, RpoN, and FliA on the regulation of the expression of transmissive traits in L. pneumophila strain Paris at different time points (replicative phase, early transmissive phase, late transmissive phase).

2009-12-02 | E-MTAB-175 | biostudies-arrayexpress

Transcription profiling of Legionella pneumophila wild type vs fleR mutant at OD 3.3

Project description:Comparison of gene expressions of Legionella pneumophila wide types and fleR mutants. Liquid culture (60 ml) BCYE, shaking, 37 ¡C. GenePix Pro for quantification with parameters: Background and Measure. BloDAP and R: adjustments - Bonferroni and Benjamini & Yekutieli; normalization - global loess; transformation - log; test - student test with varmixt; filtering - spot saturation detection. Axon for scanning. Contact: Christian Weber and Tobias Sahr

2009-11-24 | E-MTAB-163 | biostudies-arrayexpress

RNAseq profiling of a lpp0148 mutant compared to its isogenic parent Legionella pneumophila strain Paris

Project description:Comparaison of the transcriptional profile of mutant of the lpp0148 gene and the parent strain Legionella pneumophila strain Paris. lpp0148 codes for a protein with a ProQ domain (PFAM PF04352). A mutant of this gene was obtained by inserting a premature stop codon in the lpp0148 ORF. The lpp0148 mutant is constitutively competent for natural transformation as described here http://www.ncbi.nlm.nih.gov/pubmed/26526572. Transcriptional profiling was done on cultures grown to exponential phase (OD=0.8). Three independent biological replicates were analysed.

2016-05-24 | E-MTAB-4094 | biostudies-arrayexpress

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