Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Comparative genomic hybridisation of Staphylococcus aureus isolates from healthy adults

ABSTRACT: Comparative genomic hybridisation of 20 Staphylococcus aureus isolates from healthy adults.

ORGANISM(S): Staphylococcus aureus

SUBMITTER: Maria Sangvik

PROVIDER: E-MTAB-1672 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Staphylococcus aureus nasal isolates from healthy individuals cause highly variable host cell responses in vitro: the Tromsø Staph and Skin Study.

Askarian Fatemeh F Sangvik Maria M Hanssen Anne-Merethe AM Snipen Lars L Sollid Johanna U E JU Johannessen Mona M

Pathogens and disease 20131011 2

Studies on Staphylococcus aureus populations colonizing the nasal cavity reveal that some bacterial strains are more common, while others are rarely found. This study included five isolates with the most common spa types and five isolates with rare spa types from healthy population. Selected phenotypic traits and genomic content among nasal S. aureus isolates were compared. Besides the rather similar growth rates, our data revealed a high diversity among isolates; that is, in biofilm formation, ...[more]

PMID: 24115641

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Project description:The transposon site hybridization (TraSH) technique (Sassetti, CM et al. 2001. PNAS 98:12712-7) was utilized to identify genes important for the survival of Y. pestis within murine macrophages. A transposon library was created with ~31,500 Y. pestis KIM6+ insertion mutants. A portion of the Y. pestis transposon insertion mutant library was used to infect BMMs and the surviving bacteria (output pool) were recovered. TraSH was used to compare the output pool to a portion of the library that was not subjected to selection (input pool) in order to identify Y. pestis genes important for survival in macrophages. Each end of the transposon used for mutagenesis contains an outward-reading T7 RNA polymerase promoter. RNAs transcribed from the T7 promoters are complementary to the chromosomal DNA flanking each transposon in the library, so the RNAs can be used as “targets” to identify the approximate position of each transposon insertion in the mutant pool. Differentially labeled targets generated from the output and input pools are competitively hybridized to the 70-mer oligonucleotide microarrays obtained from Pathogen Functional Genomics Resource Center/J. Craig Venter Institute. Genes important for survival of Y. pestis in macrophages are identified by determining the ratio of the signal intensities for the output and input targets hybridizing to a given probe. A transposon library was created with ~31,500 Y. pestis KIM6+ insertion mutants. A portion of the Y. pestis transposon insertion mutant library was used to infect BMMs and the surviving bacteria (output pool) were recovered. TraSH was used to compare the output pool to a portion of the library that was not subjected to selection (input pool). Each end of the transposon used for mutagenesis contains an outward-reading T7 RNA polymerase promoter. RNAs transcribed from the T7 promoters are complementary to the chromosomal DNA flanking each transposon in the library, so the RNAs was used as “targets” to identify the approximate position of each transposon insertion in the mutant pool. Differentially labeled targets generated from the output and input pools are competitively hybridized to the 70-mer oligonucleotide microarrays obtained from Pathogen Functional Genomics Resource Center/J. Craig Venter Institute. Genes important for survival of Y. pestis in macrophages are identified by determining the ratio of the signal intensities for the output and input targets hybridizing to a given probe.

Dataset Information

Comparative genomic hybridisation of Staphylococcus aureus isolates from healthy adults

Publications

Staphylococcus aureus nasal isolates from healthy individuals cause highly variable host cell responses in vitro: the Tromsø Staph and Skin Study.

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