Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptome of holcus lanatus


ABSTRACT: The wild grass Holcus lanatus L., an outcrossing diploid (2n=14) and closely related to B. distachyon (Aliscioni et al., 2012), has a remarkable balanced polymorphism in arsenate tolerance, screened from a semi-natural, non-arsenic contaminated populations (Meharg et al., 1993), coded by a single gene (Macnair et al., 1992). As arsenate is a phosphate analogue it has been postulated that this polymorphism is maintained due to phosphorus nutrition, not arsenate tolerance per se, particularly as the tolerance gene co-segregates with suppression of High affinity Phosphate Transport (HAPT) (Meharg et al., 1992a; Meharg & Macnair, 1992b), though an explicit ecological link to phosphorus status of soils has yet to be proven (Naylor et al., 1996). The aim of this study is to address soil phosphate responsiveness (+/-) along with the transcriptomic consequences of being of arsenic tolerant (T) or non-tolerant (N) phenotype to ascertain why and how this polymorphism is maintained.

INSTRUMENT(S): 454 GS FLX, Illumina HiSeq 2000

ORGANISM(S): Holcus lanatus L

SUBMITTER: Caroline Meharg 

PROVIDER: E-MTAB-1678 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Trait-directed de novo population transcriptome dissects genetic regulation of a balanced polymorphism in phosphorus nutrition/arsenate tolerance in a wild grass, Holcus lanatus.

Meharg Caroline C   Khan Bayezid B   Norton Gareth G   Deacon Claire C   Johnson David D   Reinhardt Richard R   Huettel Bruno B   Meharg Andrew A AA  

The New phytologist 20130918 1


The aim of this study was to characterize the transcriptome of a balanced polymorphism, under the regulation of a single gene, for phosphate fertilizer responsiveness/arsenate tolerance in wild grass Holcus lanatus genotypes screened from the same habitat. De novo transcriptome sequencing, RNAseq (RNA sequencing) and single nucleotide polymorphism (SNP) calling were conducted on RNA extracted from H. lanatus. Roche 454 sequencing data were assembled into c. 22,000 isotigs, and paired-end Illumin  ...[more]

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