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L3MBTL2 in HEK293 cells - ChIPseq

ABSTRACT: To elucidate the (possibly sumo dependent) binding sites of L3MBTL2, wild type L3MBTL2, L3MBTL2-Flag and L3MBTL2-KR-Flag ChIPs from HEK293 cells were analyzed via ChIPseq

INSTRUMENT(S): Illumina Genome Analyzier Iix

ORGANISM(S): Homo sapiens

SUBMITTER: Florian Finkernagel

PROVIDER: E-MTAB-1731 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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SUMOylation of the polycomb group protein L3MBTL2 facilitates repression of its target genes.

Stielow Christina C Stielow Bastian B Finkernagel Florian F Scharfe Maren M Jarek Michael M Suske Guntram G

Nucleic acids research 20131224 5

Lethal(3) malignant brain tumour like 2 (L3MBTL2) is an integral component of the polycomb repressive complex 1.6 (PRC1.6) and has been implicated in transcriptional repression and chromatin compaction. Here, we show that L3MBTL2 is modified by SUMO2/3 at lysine residues 675 and 700 close to the C-terminus. SUMOylation of L3MBTL2 neither affected its repressive activity in reporter gene assays nor it's binding to histone tails in vitro. In order to analyse whether SUMOylation affects binding of ...[more]

PMID: 24369422

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