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Transcription profiling of mouse liver with CEBPa wild type and knockout animals.

ABSTRACT: Expression analysis of CEBPa knockout effects on gene expression in adult mouse liver. We used a conditional knock out strategy to delete CEBPa specifically in adult mouse hepatocytes and analysed the resulting changes in gene expression by means of microarrays.

INSTRUMENT(S): Illumina MouseWG-6 v2 Expression BeadChip

ORGANISM(S): Mus musculus

SUBMITTER: Petra Catalina Schwalie

PROVIDER: E-MTAB-178 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Five-vertebrate ChIP-seq reveals the evolutionary dynamics of transcription factor binding.

Schmidt Dominic D Wilson Michael D MD Ballester Benoit B Schwalie Petra C PC Brown Gordon D GD Marshall Aileen A Kutter Claudia C Watt Stephen S Martinez-Jimenez Celia P CP Mackay Sarah S Talianidis Iannis I Flicek Paul P Odom Duncan T DT

Science (New York, N.Y.) 20100408 5981

Transcription factors (TFs) direct gene expression by binding to DNA regulatory regions. To explore the evolution of gene regulation, we used chromatin immunoprecipitation with high-throughput sequencing (ChIP-seq) to determine experimentally the genome-wide occupancy of two TFs, CCAAT/enhancer-binding protein alpha and hepatocyte nuclear factor 4 alpha, in the livers of five vertebrates. Although each TF displays highly conserved DNA binding preferences, most binding is species-specific, and al ...[more]

PMID: 20378774

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