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Unknown,Transcriptomics,Genomics,Proteomics

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Transcriptional profiling of LIF stimulation in mouse embryonic stem cells

ABSTRACT: Stat3+/+ and -/- ES cells were cultured in defined conditions and exposed to leukemia inhibitory factor (LIF) in a 24h timecourse to identify Stat3-independent targets of the self-renewal response to LIF signaling.

INSTRUMENT(S): Illumina Genome Analyzer IIx

ORGANISM(S): Mus musculus

SUBMITTER: Paul Bertone

PROVIDER: E-MTAB-1796 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of the missing pluripotency mediator downstream of leukaemia inhibitory factor.

Martello Graziano G Bertone Paul P Smith Austin A

The EMBO journal 20130813 19

Self-renewal of pluripotent mouse embryonic stem (ES) cells is sustained by the cytokine leukaemia inhibitory factor (LIF) acting through the transcription factor Stat3. Several targets of Stat3 have previously been identified, most notably the reprogramming factor Klf4. However, such factors are neither required nor sufficient for the potent effect of LIF. We took advantage of Stat3 null ES cells to confirm that Stat3 mediates the self-renewal response to LIF. Through comparative transcriptome ...[more]

PMID: 23942233

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