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Alternative splicing profiling of human breast cancer cell lines.

ABSTRACT: We assessed alternative splicing in breast cancer through global profiling of transcriptomes of basal and luminal subtype cell lines using Affymetrix Human Junction Array.

ORGANISM(S): Homo sapiens

SUBMITTER: Anna Lapuk

PROVIDER: E-MTAB-183 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Exon-level microarray analyses identify alternative splicing programs in breast cancer.

Lapuk Anna A Marr Henry H Jakkula Lakshmi L Pedro Helder H Bhattacharya Sanchita S Purdom Elizabeth E Hu Zhi Z Simpson Ken K Pachter Lior L Durinck Steffen S Wang Nicholas N Parvin Bahram B Fontenay Gerald G Speed Terence T Garbe James J Stampfer Martha M Bayandorian Hovig H Dorton Shannon S Clark Tyson A TA Schweitzer Anthony A Wyrobek Andrew A Feiler Heidi H Spellman Paul P Conboy John J Gray Joe W JW

Molecular cancer research : MCR 20100706 7

Protein isoforms produced by alternative splicing (AS) of many genes have been implicated in several aspects of cancer genesis and progression. These observations motivated a genome-wide assessment of AS in breast cancer. We accomplished this by measuring exon level expression in 31 breast cancer and nonmalignant immortalized cell lines representing luminal, basal, and claudin-low breast cancer subtypes using Affymetrix Human Junction Arrays. We analyzed these data using a computational pipeline ...[more]

PMID: 20605923

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