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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of Acanthamoeba polyphaga mimivirus infecting Acanthamoeba castellanii for different lengths of time

ABSTRACT: A culture of Acanthamoeba castellanii cells was infected with mimivirus. At specified times after infection, infected cells aliquots were subjected to total RNA extraction. Cy3 labeled cDNA was hybridized on custom designed Agilent oligonucleotide tiling microarrays covering both strands of the mimivirus genome.

ORGANISM(S): Acanthamoeba polyphaga mimivirus

SUBMITTER: Pascal Hingamp

PROVIDER: E-MTAB-187 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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mRNA deep sequencing reveals 75 new genes and a complex transcriptional landscape in Mimivirus.

Legendre Matthieu M Audic Stéphane S Poirot Olivier O Hingamp Pascal P Seltzer Virginie V Byrne Deborah D Lartigue Audrey A Lescot Magali M Bernadac Alain A Poulain Julie J Abergel Chantal C Claverie Jean-Michel JM

Genome research 20100401 5

Mimivirus, a virus infecting Acanthamoeba, is the prototype of the Mimiviridae, the latest addition to the nucleocytoplasmic large DNA viruses. The Mimivirus genome encodes close to 1000 proteins, many of them never before encountered in a virus, such as four amino-acyl tRNA synthetases. To explore the physiology of this exceptional virus and identify the genes involved in the building of its characteristic intracytoplasmic "virion factory," we coupled electron microscopy observations with the m ...[more]

PMID: 20360389

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