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Phenotype modulation of VSMCs on patterned substrate

ABSTRACT: Loss of contractility and acquisition of an epithelial phenotype of vascular smooth muscle cells (VSMCs) are key events in proliferative vascular pathologies such as atherosclerosis and post-angioplastic restenosis. There is no proper cell culture system allowing VSMC differentiation so that it is difficult to delineate the molecular mechanism responsible for proliferative vasculopathy. We investigated whether a micro-patterned substrate could restore the contractile phenotype of VSMCs in vitro. To induce and maintain the differentiated VSMC phenotype in vitro, we introduced a micro-patterned groove substrate to modulate the morphology and function of VSMCs.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Jungsul Lee

PROVIDER: E-MTAB-1888 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Phenotypic modulation of primary vascular smooth muscle cells by short-term culture on micropatterned substrate.

Chang Soyoung S Song Seungjeong S Lee Jungsul J Yoon Jonghee J Park Junseong J Choi Sungyoung S Park Je-Kyun JK Choi Kyungsun K Choi Chulhee C

PloS one 20140204 2

Loss of contractility and acquisition of an epithelial phenotype of vascular smooth muscle cells (VSMCs) are key events in proliferative vascular pathologies such as atherosclerosis and post-angioplastic restenosis. There is no proper cell culture system allowing differentiation of VSMCs so that it is difficult to delineate the molecular mechanism responsible for proliferative vasculopathy. We investigated whether a micropatterned substrate could restore the contractile phenotype of VSMCs in vit ...[more]

PMID: 24505388

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