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Tri-KIX_DP_thymocytes

ABSTRACT: To determine the effect of mutation of the KIX domain of CBP and p300 on gene expression, thymuses dissected from four to seven week old wild type and CBP KIX/KIX;p300 +/KIX adult mice were dissociated into single cell suspesions, antibody stained for CD4 and CD8, sorted for CD4+ CD8+ double positive thymocytes, RNA was made from the sorted cells and transcriptional profiling by array was carried out. CD4+CD8+ double positive thymocytes were chosen because these cells express c-Myb.

ORGANISM(S): Mus musculus

SUBMITTER: Lawryn Kasper

PROVIDER: E-MTAB-1974 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genetic interaction between mutations in c-Myb and the KIX domains of CBP and p300 affects multiple blood cell lineages and influences both gene activation and repression.

Kasper Lawryn H LH Fukuyama Tomofusa T Lerach Stephanie S Chang Yunchao Y Xu Wu W Wu Song S Boyd Kelli L KL Brindle Paul K PK

PloS one 20131210 12

Adult blood cell production or definitive hematopoiesis requires the transcription factor c-Myb. The closely related KAT3 histone acetyltransferases CBP (CREBBP) and p300 (EP300) bind c-Myb through their KIX domains and mice homozygous for a p300 KIX domain mutation exhibit multiple blood defects. Perplexingly, mice homozygous for the same KIX domain mutation in CBP have normal blood. Here we test the hypothesis that the CBP KIX domain contributes subordinately to hematopoiesis via a genetic int ...[more]

PMID: 24340053

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