Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

HL_Arabidopsis_snrkD

ABSTRACT: Arabidopsis thaliana plants from the genotypes Col-0 and snrk2.2-2.3 (snrkD, double knock-out) were used. They were grown in a growth chamber for 5 weeks in short day (8/16 light/dark cycle) at 22 degrees, 150 umol*m2*s-1 (fluorescent lights) and 55% RH in 100-ml pots with Levingtons L2+S. On the day of the experiment, two hours after the lights turned on they were subjected to mild light stress (approx. 1500 umol*m-2*s-1, measured with a PAR light meter) for 30 min under an Isolight machine (white LED array), and immediately snap-frozen in liquid nitrogen. The outer leaves from four biological replicates were ground and RNA purified, and labelled RNA was produced with the Agilent Quick Amp kit. Labelled RNA was hybridized to Agilent Arabidopsis V4 microarray slides, and the fluorescence recorded with a Genepix scanner.

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Ruben Alvarez-Fernandez

PROVIDER: E-MTAB-2052 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Abscisic acid signalling determines susceptibility of bundle sheath cells to photoinhibition in high light-exposed Arabidopsis leaves.

Gorecka Magdalena M Alvarez-Fernandez Ruben R Slattery Katie K McAusland Lorna L Davey Phillip A PA Karpinski Stanislaw S Lawson Tracy T Mullineaux Philip M PM

Philosophical transactions of the Royal Society of London. Series B, Biological sciences 20140303 1640

The rapid induction of the bundle sheath cell (BSC)-specific expression of ASCORBATE PEROXIDASE2 (APX2) in high light (HL)-exposed leaves of Arabidopsis thaliana is, in part, regulated by the hormone abscisic acid (ABA) produced by vascular parenchyma cells. In this study, we provide more details of the ABA signalling that regulates APX2 expression and consider its importance in the photosynthetic responses of BSCs and whole leaves. This was done using a combination of analyses of gene expressio ...[more]

PMID: 24591719

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Project description:Living organisms detect seasonal changes in day length (photoperiod), and alter their physiological functions accordingly, to fit seasonal environmental changes. This photoperiodic system is implicated in seasonal affective disorders and the season-associated symptoms observed in bipolar disease and schizophrenia. Thyroid-stimulating hormone beta subunit (Tshb), induced in the pars tuberalis (PT), plays a key role in the pathway that regulates animal photoperiodism. However, the upstream inducers of Tshb expression remain unknown. Here we show that late-night light stimulation acutely triggers the Eya3-Six1 pathway, which directly induces Tshb expression. Using melatonin-proficient CBA/N mice, which preserve the photoperiodic Tshb-expression response, we performed a genome-wide expression analysis of the PT under chronic short-day and long-day conditions. These data comprehensively identified long-day and short-day genes, and indicated that late-night light stimulation induces long-day genes. We verified this by advancing and extending the light period by 8 hours, which acutely induced Tshb expression, within one day. In a genome-wide expression analysis under this condition, we searched for candidate upstream genes by looking for expression that preceded Tshb’s, and identified Eya3 gene. These results elucidate the comprehensive transcriptional photoperiodic response in the PT, revealing the complex regulation of Tshb expression and unexpectedly rapid response to light changes in the mammalian photoperiodic system. Mice were separated into 2 groups. One group was maintained under the short-day conditions (light: dark = 8 h:16 h, ZT0 = lights on, ZT8 = lights off, 400 lux) and the other was housed under long-day conditions (light:dark = 16 h:8 h, ZT0 = lights on, ZT16 = lights off, 400 lux) for 2 weeks. The PTs of both groups were retrieved every 4 h for 1 day (6 time points for each group), starting at ZT0. For the experiments performed during the first day of the long-day conditions, we applied two different conditions, following 3 weeks under short-day conditions. In one, the light-onset was advanced by 8 hours (advance condition), and in the other, the dark period was delayed by 8 hours (delay condition). PTs from both groups were obtained every 4 h for 1 day, starting at the lights-on time. (Lights on for the advance condition was ZT16 as defined by the short-day condition. Lights on for the delay condition was ZT0). We sampled 25 mice at each time point. This whole procedure was repeated twice (n = 2) to obtain experimental replicates.

Dataset Information

HL_Arabidopsis_snrkD

Publications

Abscisic acid signalling determines susceptibility of bundle sheath cells to photoinhibition in high light-exposed Arabidopsis leaves.

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