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ChiP-seq profiling of Drosophila melanogaster salivary glands to identify targets for NSL1 and MCRS2.

ABSTRACT: ChiP-seq profiling of Drosophila melanogaster salivary glands to identify targets for NSL1 and MCRS2.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Juan Vaquerizas

PROVIDER: E-MTAB-214 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The nonspecific lethal complex is a transcriptional regulator in Drosophila.

Raja Sunil Jayaramaiah SJ Charapitsa Iryna I Conrad Thomas T Vaquerizas Juan M JM Gebhardt Philipp P Holz Herbert H Kadlec Jan J Fraterman Sven S Luscombe Nicholas M NM Akhtar Asifa A

Molecular cell 20100601 6

Here, we report the biochemical characterization of the nonspecific lethal (NSL) complex (NSL1, NSL2, NSL3, MCRS2, MBD-R2, and WDS) that associates with the histone acetyltransferase MOF in both Drosophila and mammals. Chromatin immunoprecipitation-Seq analysis revealed association of NSL1 and MCRS2 with the promoter regions of more than 4000 target genes, 70% of these being actively transcribed. This binding is functional, as depletion of MCRS2, MBD-R2, and NSL3 severely affects gene expression ...[more]

PMID: 20620954

Publication: 1/2

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