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Unknown,Transcriptomics,Genomics,Proteomics

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Transcript profiling of NF-YC10-overexpressing Arabidopsis seedlings under heat stress

ABSTRACT: NF-YC10 is a subunit of the NF-Y transcription factor complex in Arabidopsis thaliana. We identified NF-YC10 as an interactor of the transcription factor DREB2A, which binds to DRE and activate expression of target genes under heat and/or dehydration stress conditions. Transgenic Arabidopsis plants that overexpress NF-YC10 showed stronger expression of DREB2A target genes under heat stress and were more tolerant to heat stress tolerance than the vector control plants. We conducted a transcript profiling by microarray aiming to identify target genes of NF-YC10 under heat stress

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Junya Mizoi

PROVIDER: E-MTAB-2144 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Arabidopsis DPB3-1, a DREB2A interactor, specifically enhances heat stress-induced gene expression by forming a heat stress-specific transcriptional complex with NF-Y subunits.

Sato Hikaru H Mizoi Junya J Tanaka Hidenori H Maruyama Kyonosin K Qin Feng F Osakabe Yuriko Y Morimoto Kyoko K Ohori Teppei T Kusakabe Kazuya K Nagata Maika M Shinozaki Kazuo K Yamaguchi-Shinozaki Kazuko K

The Plant cell 20141209 12

DEHYDRATION-RESPONSIVE ELEMENT BINDING PROTEIN2A (DREB2A) is a key transcription factor for drought and heat stress tolerance in Arabidopsis thaliana. DREB2A induces the expression of dehydration- and heat stress-inducible genes under the corresponding stress conditions. Target gene selectivity is assumed to require stress-specific posttranslational regulation, but the mechanisms of this process are not yet understood. Here, we identified DNA POLYMERASE II SUBUNIT B3-1 (DPB3-1), which was previo ...[more]

PMID: 25490919

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