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Drosophila melanogaster 4C-Seq experiments in whole embro and mesoderm cells during embryogenesis

ABSTRACT: Chromosome conformation capture (4C-Seq) in Drosophila Twist-H2B embryos (carrying nuclear tag specifically in the mesoderm) during embryogenesis was performed, anchoring on 107 different viewpoints. Two timepoints (3-4hrs and 6-8hrs after egg laying) and two tissue context (whole embryo and mesoderm) were assayed. Two independent collections were performed at each timepoint.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Charles Girardot

PROVIDER: E-MTAB-2180 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Enhancer loops appear stable during development and are associated with paused polymerase.

Ghavi-Helm Yad Y Klein Felix A FA Pakozdi Tibor T Ciglar Lucia L Noordermeer Daan D Huber Wolfgang W Furlong Eileen E M EE

Nature 20140702 7512

Developmental enhancers initiate transcription and are fundamental to our understanding of developmental networks, evolution and disease. Despite their importance, the properties governing enhancer-promoter interactions and their dynamics during embryogenesis remain unclear. At the β-globin locus, enhancer-promoter interactions appear dynamic and cell-type specific, whereas at the HoxD locus they are stable and ubiquitous, being present in tissues where the target genes are not expressed. The ex ...[more]

PMID: 25043061

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