Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

46 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Aerobic v. anaerobic gene expression in Aggregatibacter actinomycetemcomitans

ABSTRACT: The opportunistic pathogen A. actinomycetemcomitans (Aa) expereinces fluctuating oxygen levels in its natural habitat, the gingival crevice of the human oral cavity. Since oxygen influences metabolic interactions between Aa and other oral microbes, we sought to characterize oxygen-dependent Aa gene expression.

ORGANISM(S): Aggregatibacter actinomycetemcomitans

SUBMITTER: Apollo Stacy

PROVIDER: E-MTAB-2213 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Bacterial fight-and-flight responses enhance virulence in a polymicrobial infection.

Stacy Apollo A Everett Jake J Jorth Peter P Trivedi Urvish U Rumbaugh Kendra P KP Whiteley Marvin M

Proceedings of the National Academy of Sciences of the United States of America 20140513 21

The oral pathogen Aggregatibacter actinomycetemcomitans (Aa) resides in infection sites with many microbes, including commensal streptococci such as Streptococcus gordonii (Sg). During infection, Sg promotes the virulence of Aa by producing its preferred carbon source, l-lactate, a phenomenon referred to as cross-feeding. However, as with many streptococci, Sg also produces high levels of the antimicrobial hydrogen peroxide (H2O2), leading to the question of how Aa deals with this potent antimic ...[more]

PMID: 24825893

Similar Datasets

Effect of Catecholamines on the Dental Pathogen Aggregatibacter actinomycetemcomitans

2015-05-12 | GSE68749 | GEO

Effect of Catecholamines on the Dental Pathogen Aggregatibacter actinomycetemcomitans

Project description:The two-component system qseBC is a putative prokaryotic adrenergic receptor. In Aggregatibacter actinomycetemcomitans (Aa), it was shown that the qseBC responds significantly to the presence of both catecholamines and ferrous iron. In addition, growth is significantly increased in the presence of both. Therefore, we performed a custom Aa microarray in order to determine the effects of catecholamines and catecholamines and iron (CAT-Fe) on Aa. Aggregatibacter actinomycetemcomitans was grown in triplicate in a chemically defined media (CDM) supplemented with either 100uM of ferrous chloride or 50uM of norepinephrine or both. At mid-log, RNA was harvested using the Qiagen Lipid Tissue Minikit.

2015-05-12 | E-GEOD-68749 | biostudies-arrayexpress

The proteomes of oral cells alter during co-cultivation with Aggregatibacter actinomycetemcomitans and Eikenella corrodens The proteomes of oral cells alter during co-cultivation with Aggregatibacter actinomycetemcomitans and Eikenella corrodens

Project description:Although periodontitis is a widespread disease, its molecular mechanisms in human oral cells are not fully understood. Therefore, we established cell lines from the human oral cavity, including gingival keratinocytes (GK), osteoblastic lineage cells from the alveolar bone (OLAB), PDL fibroblasts (PDLF) and cementum cells (CC). Using label-free quantitative mass spectrometry, we investigated changes of the proteome of healthy human oral cells after co-cultivation with Aggregatibacter actinomycetemcomitans and Eikenella corrodens for 24 h, for the first time. Our findings show specific protein profiles for each of the human oral cell lines. This will help to understand pathologic mechanisms in periodontitis and related diseases, such as rheumatoid arthritis, diabetes mellitus or atherosclerotic vascular diseases.

2023-05-10 | PXD013919 | Pride

Connections between exoproteome heterogeneity and virulence in the oral pathogen Aggregatibacter actinomycetemcomitans

Project description:Aggregatibacter actinomycetemcomitans (Aa) is a Gram-negative bacterial pathogen associated with severe periodontitis and non-oral diseases. Clinical isolates of Aa display a rough (R) colony phenotype with strong adherent properties. Upon prolonged culturing, non-adherent strains with a smooth (S) colony phenotype emerge. To date, most virulence studies on Aa have been performed with S strains of Aa, whereas the virulence of clinical R isolates received relatively little attention. Since the extracellular proteome is the main bacterial reservoir of virulence factors, the present study was aimed at a comparative analysis of this sub-proteome fraction for a collection of R isolates and derivative S strains, in order to link particular proteins to the virulence of Aa with serotype b. To assess the bacterial virulence, we applied different infection models based on larvae of the greater wax moth Galleria mellonella, a human salivary gland-derived epithelial cell line, and freshly isolated neutrophils from healthy human volunteers. A total number of 351 extracellular Aa proteins was identified by mass spectrometry, with the S strains consistently showing more extracellular proteins than their parental R isolates. A total of 50 known extracellular virulence factors was identified, of which 15 were expressed by all investigated bacteria. Importantly, the comparison of differences in exoproteome composition and virulence highlights critical roles of 10 extracellular proteins in the different infection models. Altogether, our present study provides novel cues for understanding the virulence of Aa, and for development of potential preventive or therapeutic avenues to neutralize this important oral pathogen.

2022-06-01 | PXD028940 | Pride

Crosstalk between Streptococcus mutans and Aggregatibacter actinomycetemcomitans in dual-species biofilms.

Project description:We performed comparative analysis of transcriptomes of S. mutans in single biofilms and in mixed-biofilms with A. actinomycetemcomitans. We also compared the transcriptomic profiles of A. actinomycetemcomitans in single biofilms and A. actinomycetemcomitans in mixed biofilms with S. mutans. Finally we looked at the changes in gene expression in both organisms in time.

2017-03-31 | GSE75019 | GEO

Differential virulence of Aggregatibacter actinomycetemcomitans serotypes explained by exoproteome heterogeneity

Project description:Aggregatibacter actinomycetemcomitans (Aa) is a Gram-negative bacterial pathogen associated with periodontitis and non-oral diseases like rheumatoid arthritis and Alzheimer´s disease. Aa isolates with the serotypes a, b and c are globally most prevalent. Importantly, isolates displaying these serotypes have different clinical presentations. While serotype b isolates are predominant in severe periodontitis, serotypes a and c are generally encountered in mild periodontitis or healthy individuals. It was so far not known how these differences are reflected in the overall secretion of virulence factors. Therefore, this study was aimed at a comparative analysis of exoproteomes from different clinical Aa isolates with serotypes a, b or c by mass spectrometry, and a subsequent correlation of the recorded exoproteome profiles with virulence. Overall, we identified 425 extracellular proteins. Significant differences in the exoproteome composition of isolates with different serotypes were observed in terms of protein identification and abundance. In particular, serotype a isolates presented more extracellular proteins than serotype b or c isolates. These differences are mirrored in their virulence in infection models based on human salivary gland epithelial cells and neutrophils. Remarkably, serotype a isolates displayed stronger adhesive capabilities and induced more lysis of epithelial cells and neutrophils than serotype b or c isolates. Conversely, serotype c isolates showed relatively low leukotoxicity, while provoking NETosis to similar extents as serotype a and b isolates. Altogether, we conclude that the differential virulence presentation by Aa isolates with the dominant serotypes a, b or c can be explained by their exoproteome heterogeneity.

2022-12-16 | PXD036160 | Pride

Transcription profiling of human gingival epithelial cells responses to pathogenic oral microbial species.

Project description:Transcriptional profiling was utilized to define the biological pathways of gingival epithelial cells modulated by co-culture with the oral pathogenic Porphyromonas gingivalis and Aggregatibacter (formerly actinobacillus) actinomycetemcomitans. We used microarrays to detail the global programme of gene expression underlying infection and identified distinct classes of up- and down-regulated genes during this process. Experiment Overall Design: Gingival epithelial HIGK cells were sham infected (CTRL) and infected with either the oral pathogenic P. gingivalis (Pg) or A. actinomycetemcomitans (Aa). These samples were hybridized to Affymetrix microarrays. Understanding how host cells have adapted to pathogens, and how barrier cells respond to limit their impact, provides a mechanistic biological basis of microbial disease in the mixed bacterial-human ecosystem of the oral cavity.

2009-04-24 | E-GEOD-9723 | biostudies-arrayexpress