Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

58 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Porphyromonas gingivalis gene expression during co-culture with Treponema denticola

ABSTRACT: Porphyromonas gingivalis and Treponema denticola are periodontalpathogens that are associated with the severity and progression of periodontal diseases. This study investigates the gene expression of Porphyromonas gingivalis during co-culture with Treponema denticola

ORGANISM(S): Porphyromonas gingivalis

SUBMITTER: Kheng Tan

PROVIDER: E-MTAB-2214 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Treponema denticola gene expression during co-culture with Porphyromonas gingivalis

Project description:Porphyromonas gingivalis and Treponema denticola are periodontalpathogens that are associated with the severity and progression of periodontal diseases. this study investigates the gene expression of Treponema denticola during co-culture with Porphyromonas gingivalis.

2014-03-31 | E-MTAB-2257 | biostudies-arrayexpress

Transcription profiling of Porphyromonas gingivalis W50 cells grown in biofilm or planktonically

Project description:Transcriptomic profiling of P. gingivalis W50 biofilm grown cells to that of planktonic cells.

2009-01-31 | E-TABM-467 | biostudies-arrayexpress

Polymicrobial periodontal pathogens transcriptomes in calvarial bone and soft tissue

Project description:The objectives of this investigation were to examine changes in the host transcriptional profiles during a polymicrobial periodontal pathogens Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythia infection using a murine calvarial model of inflammation and bone resorption. P. gingivalis FDC 381, T. denticola ATCC 35404, and T. forsythia ATCC 43037 was injected into the subcutaneous soft tissue over the calvaria of BALB/c mice for 3 days, after which the soft tissues and calvarial bones were excised. RNA was isolated from infected soft tissues and calvarial bones and analyzed for transcript profiles using Murine GeneChipM-BM-. MG-MOE430A Affymetrix arrays to provide a molecular profile of the events that occur following infection of these tissues. We used mouse microarrays to detail the molecular profile of the events that occur following infection of calvarial and bone tissues and identified distinct classes of up- and down-regulated genes during this process. P. gingivalis/T. denticola/T. forsythia was injected at 5 x 108 each (N = 10) into the soft tissues overlying the calvaria of the mice for 3 days. A control group (N = 9) was injected with vehicle once daily for 3 days. Mice were euthanized 8 h after the last injection. The calvarial bones and overlying soft tissues from 5 mice in each group were excised, snap frozen in liquid nitrogen, and stored at M-bM-^@M-^S80M-BM-0C until RNA isolation. Total RNA was isolated from the frozen calvarial tissue and calvarial bone from each mouse (Polymicrobial bacteria infected and control animals, N = 5 in each group) with Trizol reagent (Invitrogen, CA). Equal amounts of RNA from samples were labeled and hybridized on a mouse GeneChip following the protocol described in the GeneChip Expression Analysis Technical manual (Affymetrix, Santa Clara, CA). After hybridization, the GeneChip arrays were stained and scanned in an Affymetrix GCS 3000 7G Scanner.

2014-01-10 | E-GEOD-29670 | biostudies-arrayexpress

Fn multispecies biofilm experiment

Project description:This study was conducted to identify whether F. nucleatum has species or group specific transcriptional responses by analyzing dual species biofilms containing either early or late colonizers and utilizing microarrays to identify altered gene expression of F. nucleatum.

2011-11-30 | E-MEXP-3058 | biostudies-arrayexpress

Td multispecies interactions

Project description:We utilized T. denticola as a model red complex organism to help determine whether red complex organisms can respond to one another upon cell-cell contact in mixed culture and the types of genes involved in such responses. We extracted RNA from combinations of T. denticola with either other P. intermedia, S. sanguinis, S. gordonii, F. nucleatum, P. gingivalis, or T. forsythia that had been centrifuged to induce cell contact, and identified the numbers and types of genes that were differentially regulated using microarrays.

2013-08-10 | E-MEXP-3059 | biostudies-arrayexpress

Transcription profiling of Porphyromonas gingivalis wild type and mutant PG1432::ermF to identify genes which have their expression influenced by PG1432

Project description:The aim of this experiment is to ascertain which genes have their expression influenced by PG1432. PG1432 encodes a putative sensor histidine kinase which is part of two component signal transduction that was highly up-regulated in P. gingivalis mature biofilm cells. PG1432 was disrupted in P. gingivalis W50 by insertion of ermF cassette. The strain W50 PG1432 mutant was designated ECR222. Two independent replicates of each strain were grown for 30 days in continuous culture in chemostats using brain heart infusion media supplemented with 0.5 mg mL-1 cysteine and 5 ï¿½~Aï¿½g mL-1 hemin. Although strain W50 formed sufficient biofilm to use for total RNA harvest and microarray analysis, strain ERC222 formed only sparse biofilm showing that disruption of PG1432 impaired biofilm formation by this mutant. We were unable to collect sufficient biofilm cells of strain ECR222 therefore the microarray analysis total RNAs were harvested only from planktonic phase cells. The transcriptome of the mutant ECR222 were compared to that of strain W50.

2010-01-11 | E-TABM-546 | biostudies-arrayexpress

Molecular Characterization of Treponema denticola infection-induced bone and soft tissue transcriptional profiles

Project description:The objectives of this investigation were to examine changes in the host transcriptional profiles during a Treponema denticola infection using a murine calvarial model of inflammation and bone resorption. T. denticola ATCC 35404 was injected into the subcutaneous soft tissue over the calvaria of BALB/c mice for 3 days, after which the soft tissues and calvarial bones were excised. RNA was isolated from infected soft tissues and calvarial bones and analyzed for transcript profiles using Murine GeneChip® MG-MOE430A Affymetrix arrays to provide a molecular profile of the events that occur following infection of these tissues. We used mouse microarrays to detail the molecular profile of the events that occur following infection of calvarial and bone tissues and identified distinct classes of up- and down-regulated genes during this process. T. denticola was injected at 1.5 x 109 (N = 10) into the soft tissues overlying the calvaria of the mice for 3 days. A control group (N = 9) was injected with vehicle once daily for 3 days. Mice were euthanized 8 h after the last injection. The calvarial bones and overlying soft tissues from 5 mice in each group were excised, snap frozen in liquid nitrogen, and stored at –80°C until RNA isolation. Total RNA was isolated from the frozen calvarial tissue and calvarial bone from each mouse (T. denticola infected and control animals) with Trizol reagent (Invitrogen, CA). Equal amounts of RNA from samples were labeled and hybridized on a mouse GeneChip following the protocol described in the GeneChip Expression Analysis Technical manual (Affymetrix, Santa Clara, CA). After hybridization, the GeneChip arrays were stained and scanned in an Affymetrix GCS 3000 7G Scanner.

2010-11-16 | E-GEOD-19855 | biostudies-arrayexpress

Transcription profiling of Treponema denticola biofilms vs cells grown planktonically

Project description:Comparison of T. denticola cells grown as a biofilm to cells grown planktonically

2009-12-01 | E-MEXP-2266 | biostudies-arrayexpress

Transcriptome of A. pleuropneumoniae biofilms cultured under different growth conditions

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-05-20 | E-GEOD-43824 | biostudies-arrayexpress

Hepatocytes induces properties of wound healing type and regulatory macrophages in co-cultivated BMDM

Project description:The liver comprises the body´s largest pool of macrophages constituting approximately 80 % of all sessile tissue macrophages of the body. After liver damage monocyte-derived macrophages are recruited into the liver and were here affected by a micro milieu which is considerably influenced by hepatocytes. Up to now the complex network that determines the differentiation and function of liver macrophages and the impact of the intercellular communication between macrophages and the other parenchymal and non-parenchymal cell types of the liver is not well understood. The present study investigates the role of the intercellular communication between macrophages and hepatocytes on macrophage differentiation and function. Under physiological conditions the sinusoidal endothelial cell layer and the space of Dissé separate macrophages and hepatocytes from each other. Therefore the study focuses on the impact of the intercellular communication via soluble mediators on the differentiation of bone marrow derived macrophages (BMDM), which were recruited to the liver after liver injury, in co-culture models with highly purified primary murine hepatocytes embedded in a sandwich collagen matrix.

2023-07-07 | PXD012136 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data