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Ribosome profiling upon overexpression of the RNA-binding protein Unkempt

ABSTRACT: Ribosome profiling to assess changes in ribosome occpancy upon overexpression of the RNA-binding protein Unkempt (most likely acts as a translational regulator) in human HeLa cell lines.

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER: Kathi Zarnack

PROVIDER: E-MTAB-2278 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Control of a neuronal morphology program by an RNA-binding zinc finger protein, Unkempt.

Murn Jernej J Zarnack Kathi K Yang Yawei J YJ Durak Omer O Murphy Elisabeth A EA Cheloufi Sihem S Gonzalez Dilenny M DM Teplova Marianna M Curk Tomaž T Zuber Johannes J Patel Dinshaw J DJ Ule Jernej J Luscombe Nicholas M NM Tsai Li-Huei LH Walsh Christopher A CA Shi Yang Y

Genes & development 20150301 5

Cellular morphology is an essential determinant of cellular function in all kingdoms of life, yet little is known about how cell shape is controlled. Here we describe a molecular program that controls the early morphology of neurons through a metazoan-specific zinc finger protein, Unkempt. Depletion of Unkempt in mouse embryos disrupts the shape of migrating neurons, while ectopic expression confers neuronal-like morphology to cells of different nonneuronal lineages. We found that Unkempt is a s ...[more]

PMID: 25737280

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