Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of human and mouse organism parts where organism parts are exactly matched between the two species

ABSTRACT: Human and mouse organism part comparison data set in which organism parts are exactly matched between the two species. Data obtained from E-AFMX-4 and E-AFMX-5.

ORGANISM(S): Mus musculus

SUBMITTER: John Walker

PROVIDER: E-MTAB-24 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

A gene atlas of the mouse and human protein-encoding transcriptomes.

Su Andrew I AI Wiltshire Tim T Batalov Serge S Lapp Hilmar H Ching Keith A KA Block David D Zhang Jie J Soden Richard R Hayakawa Mimi M Kreiman Gabriel G Cooke Michael P MP Walker John R JR Hogenesch John B JB

Proceedings of the National Academy of Sciences of the United States of America 20040409 16

The tissue-specific pattern of mRNA expression can indicate important clues about gene function. High-density oligonucleotide arrays offer the opportunity to examine patterns of gene expression on a genome scale. Toward this end, we have designed custom arrays that interrogate the expression of the vast majority of protein-encoding human and mouse genes and have used them to profile a panel of 79 human and 61 mouse tissues. The resulting data set provides the expression patterns for thousands of ...[more]

PMID: 15075390

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Project description:In Arabidopsis thaliana, the immediate early response of plants to cytokinin is formulated as the multistep AHK-AHP-ARR phosphorelay signaling circuitry, which is initiated by the cytokinin-receptor histidine protein kinases. In the hope of finding components (or genes) that function downstream of the cytokinin-mediated His-Asp phosphorelay signaling circuitry, we carried out genome-wide microarray analyses. To this end, we focused on a pair of highly homologous ARR10 and ARR12 genes by constructing an arr10 arr12 double null mutant. The mutant alleles used in this study were arr10-5 and arr12-1. arr10-5 is the SALK_098604 T-DNA insertion line, whose mutation was determined to be located in the fifth exon of the ARR10 coding sequence. Arr12-1 is the SALK_054752 T-DNA insertion line, whose mutation was determined to be located in the third exon of the ARR12 coding sequence. The resulting mutant exhibits a characteristic phenotype with regard to the cytokinin-mediated His-Asp phosphorelay. Here we, therefore, compared response to cytokinin in wild type with that in arr10 arr12 double mutant. In this study, wild type and the arr10 arr12 double mutant grown vertically on MS agar plates for 2 weeks were treated with 20uM t-zeatin or 0.02% DMSO (solvent for t-zetion solution) for 1h. These treated plant samples were divided into three portions, from which RNA samples were prepared separately from aerial parts of seedlings with use of RNeasy Plant Mini Kit (Qiagen, Valencia, CA, U.S.A.). The Quality of RNAs prepared was analyzed by Bioanalyzer 2100 (Agilent Technologies). These RNA samples were processed as recommended by the Affymetrix instruction (Affymetrix GeneChip Expression Analysis Technical Manual, Affymetrix). These dataset will provide us with bases for understanding the early response to cytokinin on aerial parts of seedlings in Arabidopsis thaliana. Experimenter name: Hitoshi SAKAKIBARA; Experimenter phone: 81455039576; Experimenter fax: 81455039609; Experimenter address: Biodynamics Research Team; Experimenter address: RIKEN Plant Science Center; Experimenter address: 1-7-22 Suehiro, Tsurumi; Experimenter address: Yokohama; Experimenter zip/postal_code: 230-0045; Experimenter country: JAPAN Experiment Overall Design: 12 samples were used in this experiment

Dataset Information

Transcription profiling of human and mouse organism parts where organism parts are exactly matched between the two species

Publications

A gene atlas of the mouse and human protein-encoding transcriptomes.

Similar Datasets

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