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Transcription profiling of peripheral blood leukocyte subsets in autoimmune/autoinflammatory disease patients and healthy controls

ABSTRACT: Gene expression microarray profiling was performed on peripheral blood leukocyte subsets (CD4+ T cells, CD14+ monocytes, and CD16+ neutrophils) from healthy controls and patients with flaring autoimmune disease.

ORGANISM(S): Homo sapiens

SUBMITTER: Arianne Richard

PROVIDER: E-MTAB-2452 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Comparison of gene expression microarray data with count-based RNA measurements informs microarray interpretation.

Richard Arianne C AC Lyons Paul A PA Peters James E JE Biasci Daniele D Flint Shaun M SM Lee James C JC McKinney Eoin F EF Siegel Richard M RM Smith Kenneth G C KG

BMC genomics 20140804

<h4>Background</h4>Although numerous investigations have compared gene expression microarray platforms, preprocessing methods and batch correction algorithms using constructed spike-in or dilution datasets, there remains a paucity of studies examining the properties of microarray data using diverse biological samples. Most microarray experiments seek to identify subtle differences between samples with variable background noise, a scenario poorly represented by constructed datasets. Thus, microar ...[more]

PMID: 25091430

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