Gene expression profiling of growth on methanol in a typical fed batch culture of Pichia pastoris
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ABSTRACT: We have followed a typical fed-batch induction regime for heterologous protein production under the control of the AOX1 promoter using both microarray and metabolomic analysis. The genetic constructs involved 1 and 3 copies of the TRY1 gene, encoding human trypsinogen, In small-scale laboratory cultures. This facilitated both a progressive time course analysis for each strain and side by side comparisons between wild-type and recombinant strains at the same times post-induction.
ORGANISM(S): Pichia pastoris
SUBMITTER: Geraint Barton
PROVIDER: E-MTAB-2530 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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