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A histone H3K36 chromatin switch coordinates DNA double-strand break repair pathway choice.

ABSTRACT: Wildtype and set2delta strains, with samples taken at t=0 and following 30 minutes treatment with 5 µg/ml bleomycin.

INSTRUMENT(S): Axon GenePix 4000B scanning hardware

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Lakxmi Subramanian

PROVIDER: E-MTAB-2549 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A histone H3K36 chromatin switch coordinates DNA double-strand break repair pathway choice.

Pai Chen-Chun CC Deegan Rachel S RS Subramanian Lakxmi L Gal Csenge C Sarkar Sovan S Blaikley Elizabeth J EJ Walker Carol C Hulme Lydia L Bernhard Eric E Codlin Sandra S Bähler Jürg J Allshire Robin R Whitehall Simon S Humphrey Timothy C TC

Nature communications 20140609

DNA double-strand break (DSB) repair is a highly regulated process performed predominantly by non-homologous end joining (NHEJ) or homologous recombination (HR) pathways. How these pathways are coordinated in the context of chromatin is unclear. Here we uncover a role for histone H3K36 modification in regulating DSB repair pathway choice in fission yeast. We find Set2-dependent H3K36 methylation reduces chromatin accessibility, reduces resection and promotes NHEJ, while antagonistic Gcn5-depende ...[more]

PMID: 24909977

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