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Unknown,Transcriptomics,Genomics,Proteomics

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RNA-seq of control siRNA, p150 siRNA and p60 siRNA transfected mES cells

ABSTRACT: Totipotent cells that resemble 2-cell stage embryos (2C-like cells) can arise with a very low frequency in ES cell cultures. Here we show that totipotent-like cells can be induced in vitro through the down-regulation of the chromatin assembly activity of CAF-1. Endogenous retroviruses and 2-cell stage specific genes are among the highest upregulated genes upon CAF-1 knockdown and the emerging totipotent-like cells exhibit the molecular characteristics of endogenous 2C-like cells and 2-cell embryos. CAF-1 depletion leads to increased accessibility at MERVL elements and to the up-regulation of neighbouring genes, thereby generating a transcriptional profile similar to that of 2-cell stage embryos.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

SUBMITTER: Juan Vaquerizas

PROVIDER: E-MTAB-2684 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Early embryonic-like cells are induced by downregulating replication-dependent chromatin assembly.

Ishiuchi Takashi T Enriquez-Gasca Rocio R Mizutani Eiji E Bošković Ana A Ziegler-Birling Celine C Rodriguez-Terrones Diego D Wakayama Teruhiko T Vaquerizas Juan M JM Torres-Padilla Maria-Elena ME

Nature structural & molecular biology 20150803 9

Cellular plasticity is essential for early embryonic cells. Unlike pluripotent cells, which form embryonic tissues, totipotent cells can generate a complete organism including embryonic and extraembryonic tissues. Cells resembling 2-cell-stage embryos (2C-like cells) arise at very low frequency in embryonic stem (ES) cell cultures. Although induced reprogramming to pluripotency is well established, totipotent cells remain poorly characterized, and whether reprogramming to totipotency is possible ...[more]

PMID: 26237512

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