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Glucocorticoid receptor profiling in U2OS cells expressing GRalpha or GRgamma isoform

ABSTRACT: To determine whether insertion of a single amino acid had an effect on Glucocorticoid receptor (GR) occupancy, we performed ChIP-seq of GRalpha and GRgamma in the same U2OS osteosarcoma cell lines, upon glucocorticoid treatment. These assays are also part of submission E-MTAB-2955

INSTRUMENT(S): Illumina Genome Analyzer II

ORGANISM(S): Homo sapiens

SUBMITTER: Morgane Thomas-Chollier

PROVIDER: E-MTAB-2731 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A naturally occurring insertion of a single amino acid rewires transcriptional regulation by glucocorticoid receptor isoforms.

Thomas-Chollier Morgane M Watson Lisa C LC Cooper Samantha B SB Pufall Miles A MA Liu Jennifer S JS Borzym Katja K Vingron Martin M Yamamoto Keith R KR Meijsing Sebastiaan H SH

Proceedings of the National Academy of Sciences of the United States of America 20131014 44

In addition to guiding proteins to defined genomic loci, DNA can act as an allosteric ligand that influences protein structure and activity. Here we compared genome-wide binding, transcriptional regulation, and, using NMR, the conformation of two glucocorticoid receptor (GR) isoforms that differ by a single amino acid insertion in the lever arm, a domain that adopts DNA sequence-specific conformations. We show that these isoforms differentially regulate gene expression levels through two mechani ...[more]

PMID: 24127590

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