Project description:Goal of the study is to characterize the function of OsTB1 in rice. we generated mutant lines for OsTB1 gene. We harvested the basal node containing axillary buds from the mutants as well as those of the corresponding controls. We compared the gene expression of the mutants and the controls to estimate the downstream gene regulated by the OsTB1 gene.
Project description:To understand the downstream genes of TCP13, we performed analysis of gene expression using vector control plants and the TCP13 fused repression domain plants (TCP13pro::TCP13SRDX) . TheTCP13pro::TCP13SRDX plants is transgenic plants expressing chimeric repressor (SRDX) fused to TCP13 cDNA using the TCP13 promoter. TCP13 is a drought-inducible gene and play an essential role in leaf morphology. Arabidopsis plants were grown on the Murashige and Skoog (MS) medium (Murashige and Skoog, 1962), supplemented with 3% sucrose and 0.8 % agar (under a 16-h-day (60 ± 10 μmol photons m−2 s−1 light intensity) and 8-h-night regime for 2 weeks.
Project description:Goal of the study is to characterize distinct function(s) of two cytosolic glutamine synthetase (GS) in rice plants. We grew rice lacking GS1;1 and GS1;2 under the ammonium sufficient condition. We harvested roots from the two mutants as well as those of the corresponding control.
Project description:To characterize plant HS-responsive genes, the transcriptome analysis of rice was conducted using microarray. Arabidopsis plants were grown in plastic pots filled with peat moss for 3 weeks (principal growth stage 1.07â1.08) under a 16 h light/8 h dark photoperiod (50 ± 10 μmol photons mâ2 sâ1) at 22°C, and were treated for 30 min at 37°C.
Project description:To characterize plant heat stress-responsive genes and to clarify the heat stress-responsive transcription pathways, the transcriptome analysis of rice was conducted using microarray. Rice (Oryza sativa L. cv. Nipponbare) were grown in plastic pots filled with nutrient soil for 2 weeks with a 12 h light (28°C)/12 h dark (25°C) regimen (ca. 1500 μmol photons mâ2 sâ1) and were treated for 30 min at 42°C.
Project description:To characterize heat stress-responsive genes and to clarify the heat stress-responsive transcription pathways, transcriptome analysis of soybean was conducted using microarray. Soybean (Glycine max cv. Williams82) were grown in plastic pots filled with nutrient soil for 2 weeks with a 12 h light (28°C)/12 h dark (25°C) regimen (ca. 1500 μmol photons mâ2 sâ1) and were treated for 30 min at 42°C.
Project description:To characterize plant heat stress-responsive genes and to clarify the heat stress-responsive transcription pathways, the transcriptome analysis of maize was conducted using microarray. Maize (Zea mays cv. B73) were grown in plastic pots filled with nutrient soil for 2 weeks with a 12 h light (28°C)/12 h dark (25°C) regimen (ca. 1500 μmol photons mâ2 sâ1) and were treated for 30 min at 42°C.